DIAZO-SAFRANIN FOR STAINING ENTEROCHROMAFFIN

The utilization of yolk platelets by tissues of Xenopus embryos studied by a safranin staining method.

Studies of the Common Aerobic Spore-forming Bacilli: I. Staining for Fat with Sudan Black B-safranin.

In a classification of the aerobic spore-forming bacilli proposed by Smith and Clark (1937) one of the important differential points is the presence of reserve fat in the cells of organisms of the "B. cereus-B. megatherium group" when grown on glucose agar. In contrast, bacilli of the "B. subtilis-B. mesentericus group," and some other sub-groups, are said not to store fat. The authors do not d...

Discussion: Enterochromaffin-Like Cell Pathobiology

The regulation of gastric acid secretion is a complex system, which has been elucidated over a number of years. The significance of the primary role of histamine as a stimulant of the parietal cell was proven conclusively by the demonstration of the efficacy of H2-receptor blockers [1]. The involvement of the vagal and neural system, primarily via muscarinic receptors, appears important, but it...

Use of Safranin O for Characterization of Brucella Species.

Moreira-Jacob (J. Bacteriol. 86:599, 1963) reported that 44 strains of Brucella suis were inhibited by safranin 0 in Brucella Agar (Albimi), whereas nearly all of 320 other strains of Brucella were not inhibited. Brinley-Morgan (World Health Organization, Brucellosis document , 246, 1963) examined the growth of more than 80 cultures on serum dextrose agar with added safranin 0, and found that a...

Optimized diazo scaffold for protein esterification.

The O-alkylation of carboxylic acids with diazo compounds provides a means to esterify carboxylic acids in aqueous solution. A Hammett analysis of the reactivity of diazo compounds derived from phenylglycinamide revealed that the (p-methylphenyl)glycinamide scaffold has an especially high reaction rate and ester/alcohol product ratio and esterifies protein carboxyl groups more efficiently than ...